纳米珍珠粉对大鼠深Ⅱ°烧伤创面愈合的影响(2)

Tab.2  Changement of the scalded skin tissue HP content in different phases  (x±s，n=10) 

Normal control value：78.05±7.79；t test：compared with normal control：△P＜0.05，△△P＜0.01；compared with experimental control*P＜0.05，**P＜0.01；compared with micro pearl powder：☆P＜0.05，☆☆P＜0.01

    3  Discussion
   
    By the measurement of the wound healing rate，nano pearl powder had the function of promoting obviously the wound healing in earlier period，it’s curative effect is obvious better than that of micron pearl powder by comparison (P＜0.05).The scald healing is a compound process of inflammatory reaction，cell proliferation，re-epithelization and collagen metabolizing［2］.The collagen synthesized and secreted by fibroblasts is the main structure protein of the skin，and HP is a steady component of collagen，which accounts for 13.4% of the collagen proteins.The healing of a burn lesion mainly relies on the collagen fiber formed by the granulation tissue，thus，measurement of the variance of skin HP content may act as a specific and sensitive marker for wound healing in tested animals［3］.
   
    The decrease of HP in the early stage of scald has much to do with the collagen decomposing caused by heat damage and the eschar formed by collagen degeneration［4］.The dermal HP content in the nano peal powder group started to increase on day 5，reached the same value as the normal control group on day 10，and arrived a peak value on day 15.Between day 3 and 21，the dermal HP content in the nano pearl powder group is significantly higher than those in micro pearl powder group and normal healing group，which indicates that nano peal powder has a more potent stimulation effect on collagen synthesis and a higher absorption rate than micro pear powder.So，nano peal powder can promote the healing process of a scalded tissue by relieving the decrease of HP in the affected skin and accelerating the synthesis of collagen fiber. 

（科教作文网http://zw.ΝsΕAc.com发布）
Fig1a''1b  The trauma of deep Ⅱ°degree wound in SD rats(×100)

【参考文献】

    1  YANG Tai-mei，FENG Shi-jie，XU Sa-ying.Making the rat scald model.Acta Universitatis Medicinalis Secondae Shanghai，1997，173)：91.    
    2  Portera CA，Love EJ，Memore L，et al.Effect of macrophagge stimulation on collagen biosynthesis in the healing wound.Am Surg，1997，63(2)：125-131.    
    3  Edwards CA，Orien JR.Modified assay for determination of hydroxyproline in tissue hydrolyzate.Clin Chem Acta，1980，104：161.    
    4  Nussbaum MS.Arginine stimulates wound healing and immune function in elderly humen beings.J PEN，1994，18：194.