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脊髓爆震伤后早期脊髓前角运动神经元的形态学

2014-05-17 01:15 作者：于杰，罗卓荆，张雪非，徐新智，吕荣，陈良为，耿丹
【关键词】 ,脊髓损伤
Early morphological changes of anterior horn motor neurons after explosive injury of spinal cord
　　【Abstract】 AIM: To establish an animal model with explosive injury of spinal cord, and to explore the early morphological changes of anterior horn motor neurons after explosive injury of spinal cord. METHODS: Thirtysix rabbits were randomly divided into control group （Group A, n=12）， 6 h experimental group (Group B, n=12) and 24 h experimental group (Group C, n=12). Group B and C were injured with 0.9 g cyclotrimethylene trinitramine, and 6 h and 24 h after trauma, the injured spinal cords were taken for observation of the morphological changes and apoptosis of spinal motor neurons using HE staining， toluidine blue staining and Cajal staining under light microscopes. RESULTS: The reversible change of the neurons of the rabbits happened after 6 h， and some neurons was observed dead，the mean of the death being 12.58±2.23； 24 h after trauma， a great quantity of neurons were observed dead， the mean of the death being 31.52±2.69, which differed significantly from that in the control group (P