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悬饮宁方对SPC论文

2015-05-13 01:41 作者：徐振晔，刘建文，周美云
【关键词】 悬饮宁方
［摘要］ 目的：观察中药悬饮宁方抑制人肺腺癌细胞SPCA1浸润的作用及对S180腹水瘤小鼠腹膜病理形态学变化的影响。方法：在进行小鼠腹膜间皮细胞分离培养的基础上，将SPCA1人肺腺癌细胞调整加入复层培养，观察形成的克隆数。取出S180腹水瘤小鼠腹膜，用透射电镜观察悬饮宁方各剂量组小鼠腹膜病理形态学的变化。结果：体外实验显示加入含有悬饮宁生药的培养液(50 μg/ml)后，SPCA1在琼脂培养皿背面形成的癌细胞克隆数明显减少。用悬饮宁治疗S180腹水瘤小鼠后，取出腹膜，在电镜下可观察到，从低剂量开始即可见小鼠腹膜间皮细胞排列整齐，随着剂量的递增这种现象更明显，间皮细胞排列更加整齐，增厚；而生理盐水组小鼠腹膜间皮细胞排列疏松，坏死。结论：中药悬饮宁方有抑制SPCA1细胞浸润的作用，悬饮宁还可以改变S180腹水瘤小鼠排列疏松的腹膜间皮细胞，从而控制腹水瘤小鼠癌性积液生长。
　　［关键词］ 悬饮宁方; SPCA1细胞; 间皮细胞; S180腹水瘤; 小鼠
　　Effects of Xuanyinning Recipe on invasion of SPCA1 cells and pathomorphological changes of peritoneum in mice inoculated with sarcoma 180
　　ABSTRACT Objective: To observe the effects of Xuanyinning Recipe (XYNR) in inhibiting SPCA1 cellular infiltration and on the pathomorphological changes of peritoneum in mice inoculated with sarcoma 180 (S180). Methods: On the bases of isolated culture of mouse peritoneal mesothelial cells, we adjusted and added the human lung adenocarcinoma cell line SPCA1 into the doublelayer culture medium to observe the number of clones formed. We also took out the peritoneum from the mice administered with three different dosages of XYNR and observed its pathomorphological changes with transmission electron microscope. Results: In the in vitro experiment, the number of clones of SPCA1 in culture medium with XYNR (50 μg/ml) decreased distinctly. In the in vivo experiment, it was observed that, in the peritoneum from the XYNRtreated mice inoculated with S180, the mesothelial cells arranged more and more regularly with the increasing of the dosage of XYNR, while the mesothelial cells in the peritoneum of the mice in the control group necrosed and arranged loosely. Conclusion: XYNR can inhibit the invasion of SPCA1 cells. It also can improve the loose arrangement of the peritoneal mesothelial cells in mice inoculated with S180, so as to inhibit the malignant effusion. （转载自http://www.ＮＳＥＡＣ.com中国科教评价网）
　　KEY WORDS Xuanyinning Recipe; SPCA1 cells; mesothelial cells; sarcoma 180; mice
　　研究资料表明，大约50%的癌症转移患者最终发生癌性积液［1］。而癌性积液的出现常常预示着疾病的进展和生活质量的下降，表明疾病进入晚期，生存时间4～6个月［2］。我们在临床及实验中观察到中药悬饮宁方能有效地控制癌性积液生长［3］，为了进一步探讨悬饮宁方抑制癌性积液生长的作用机制，我们进行了悬饮宁方对SPCA1细胞浸润作用的体外实验，并通过电镜观察该方对S180腹水瘤小鼠腹膜组织病理形态的影响，现报告如下。
　　1 材料与方法
　　1.1 主要仪器与试剂 超净工作台，上海大华医疗仪器厂生产；CO2培养箱，日本平尺株式会社生产；倒置相差显微镜，日本Olympus公司制造；达氏修正依氏培养基（Dulbecco’s modified Eagle’s medium, DMEM）培养粉，由日本制药株式会社生产；表皮生长因子(epidermal growth factor,EGF)，由上海细胞生物学研究所提供；胰酶、酚红、琼脂粉、35 mm培养皿均由中国科学院实生细胞生物技术公司提供；顺氯氨铂，由中国齐鲁制药厂生产(批号：9386162)，规格20 mg／瓶；S180腹水瘤细胞株，引自中国科学院上海分院药物研究所。