银杏叶提取物对百草枯致PC12细胞损伤的保护作用
2015-07-20 01:11
导读：药学论文毕业论文，银杏叶提取物对百草枯致PC12细胞损伤的保护作用怎么写，格式要求，写法技巧，科教论文网展示的这篇论文是很好的参考：作者：康小刚，陈建宗，王百忍，徐震，李海龙，奚淑芳 【关键词】 银杏叶
作者：康小刚，陈建宗，王百忍，徐震，李海龙，奚淑芳
【关键词】 银杏叶提取物
Neuroprotective effects of ginkgo biloba extract against paraquatinduced PC12 cell line damage and its mechanism
【Abstract】 AIM: To study the neuroprotective effect and related mechanisms of ginkgo biloba extract (EGB761) against paraquat(PQ) induced apoptosis in pheochromocytoma (PC12) cells. METHODS: Using PQinduced PC12 cells damage as the model of Parkinsons disease in vitro, the cytotoxicity of PQ was measured by 3［4, 5dimethylthiazol2yl］2, 5diphenyltetrazolium bromide (MTT) assay, the impairment of PC12 cells was detected by lactate dehydrogenase (LDH) assay, the apoptosis of PC12 cells was detected by flow cytometry and the morphological change of PC12 cells was observed by caspase3 immunocytochemical staining. RESULTS: 40 mg/L EGB761 played a protective role in the damage induced by 300 μmol/L PQ for 24 h. EGB761 significantly inhibited LDH release induced by PQ in PC12 cells （0.25±0.01）(P
