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针刺对抑郁症模型大鼠海马区单胺类神经递质的

2014-06-27 01:14 针刺对抑郁症模型大鼠海马区单胺类神经递质的影响 【摘要】目的 观察针刺对抑郁症模型大鼠的作用机制。方法 选择Open-Field法评分相近的40只成年SD雄性大鼠随机分为正常对照组、模型组、药物组、针刺组，每组各10只。应用分养和长期不可预见性中等强度应激造成抑郁症模型大鼠。以Open-Field法对照研究针刺对慢性应激诱导的抑郁症模型大鼠行为学的改变；应用高效液相电化学检测仪检测单胺类神经递质。结果 针刺组大鼠，在治疗第7天的行为学评分与正常对照组之间已经无明显差异（P＞0.05）；在治疗21天时，与药物氟西汀组行为学评分无明显差异（P＞0.05），与模型对照组大鼠差异存在显著性（P＜0.01）。而在治疗21天后，针刺组大鼠海马内5-HT和NE的含量，与正常组差异无显著性（P＞0.05）；与模型对照组比较，差异存在显著性（P＜0.05，P＜0.01）。结论 针刺治疗抑郁症模型大鼠的行为学异常，其疗效与氟西汀相当；抑郁症模型大鼠海马区5-HT和NE的含量与Open-field评分存在相似性；针刺疗效与海马区单胺类神经递质的改变有关系。

　　【关键词】针刺治疗；抑郁症；疾病模型；大鼠；行为学；5-HT；NE

　　Effects of acupuncture on serotonin and norepinephrine in hippocampus of depressed model rats

　【Abstract】Objective To observe how acupuncture affects the mechanism of depression model rats.Methods Using Open-Field method to randomly select 40 adult SD male rats into normal control, model group, drug treatment and acupuncture treatment group, 10 rats per group. Separation, long term unpredictability, medium stimulation techniques were applied to modify rats behavior. Then using Open-Field method to reflect changes in depressed model rats behavior between groups treated with acupuncture and control. And the concentration of monoamine neurotransmitters and their metabolites in hippocampus of rat brain were determined by HPLC-ECD. Results Compared with normal control, acupuncture treatment group’s behavior was not different （P＞0.05）on the 7th day after treatment. On the 21th day after treatment, acupuncture treatment group’s behavior was not different （P＞0.05）with drug treatment group, and was more different with model group（P＜0.01）. Compared with normal group on the 21th day of treatment, the concentration of serotonin and norepinephrine in hippocampus of acupuncture treatment group was not different （P＞0.05）, whereas was more different with model group （P＜0.05，P＜0.01）.Conclusion This experiment proved that acupuncture treatment could change the behavior abnormality of depressive model rats. The curative effects is comparable to flouxetine. The changes of the concentration of serotonin and norepinephrine in hippocampus are one of the mechanism.

　　【Key words】acupuncture treatment；depression；disease model；rat；behavior；5 hydroxytryptamine (5-HT);NE

　　自1984年，针灸开始介入抑郁症的治疗以来，取得了确切的临床疗效，但是对于针刺作用的过程和机制研究尚不深入。为了探讨针刺在抑郁症治疗中的应用规律，我们观察了针刺对抑郁症模型大鼠海马区内单胺类神经递质的影响，并与氟西汀（选择性5-羟色胺再摄取抑制剂）作对照。现将研究结果报告如下。