评价网 > 科教论文 > 医学毕业论文 > 医学论文 > 正文

TPA对人食管癌Eca109细胞增殖和细胞核形态的影响

2014-09-16 02:10 TPA对人食管癌Eca109细胞增殖和细胞核形态的影响

作者:郭玉忠，贾琳静，郜 娜，田 鑫，谢 敏，张莉蓉，乔海灵

【摘要】 目的 研究佛波酯 (12-O-tetradecanoylphorbol-13-acetate，TPA) 对人食管癌细胞的影响。方法 细胞增殖试验采用MTT法和细胞计数的方法，细胞核形态的测量采用计算机辅助的图像分析系统，分别测量计算细胞核面积(NA)、核周长(NP)、最大直径(Dmax)、 最小直径(Dmin)、核浆比(NPA)、核轴比(NAR)、核形状因子(NRF)等。结果 TPA没有抑制人食管癌Eca109细胞增殖和改变细胞核形态。 结论 TPA对人食管癌Eca109细胞可能没有促分化作用。

【关键词】 佛波酯；食管癌细胞；细胞核形态

Effects of TPA on proliferation and nuclear morphometry of human esophageal cancer Eca109 cells

【Abstract】 Objectives To investigate the effects of 12-O-tetradecanoy lphorbol-13-acetate (TPA) on human esophageal cancer Eca109 cells. Methods The sensitivity of TPA to Eca109 cells was determined with MTT assay and counting cell numbers. After the cells were stained with HE，the measurements of nuclear morphometry were performed with computer-assisted image analysis system. Results TPA did not inhibit the cells proliferation and decrease the nuclear size. Conclusions TPA may not induce Eca109 cells to differentiate.

【Key words】 12-O-tetradecanoylphorbol-13-acetate (TPA)；esophageal cancer cell line；nuclear morphometry

Human esophageal cancer is one of the most common solid tumors in China. Due to its high metastatic potential and the frequent onset of resistance to chemotherapy，it is one of the major causes of death by neoplasia in the country. The present chemotherapy of cancer uses agents that are usually toxic to normal cells. On the other hand，induction of cellular differentiation may supplement the use of non-cytotoxic drugs in several forms of neoplasia，like the successful use of all-transretinoic acid (ATRA) in the treatment of acute promyelocytic leukemia.

Plant diterpine phorbol esters，particularly the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA)，are among the most potent tumor promoters known and their effects range from stimulating DNA synthesis through activating gene expression to producing alterations in membrane properties and constituents. TPA can also potentiate the differentiation of various tumor cells in culture and in vivo of human beings. TPA can induce differentiation and inhibit the growth of a number of malignant cell types，including myeloid leukemia［1］，breast［2］，prostate［3］，colon［4］，and brain［5］. Other report showed that TPA could not induce human hepatoca


rcinoma cell line to differentiation［6］. Because of its efficiency in vivo［7］，we are interested in its effects on human esophageal cancer cells. Here，we report the effects of TPA on proliferation and nuclear morphometry of human esophageal cancer Eca109 cells.

1 Materials and methods

1.1 Chemicals TPA purchased from Sigma Chemical Co. was dissolved in ethanol and stored at -20℃.The stock solutions were diluted with Hanks’ buffered salt solution. The final concentration of ethanol in the incubation mixtures was 0.1% or less.