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【摘要】 目的 探讨不同剂量的蕨麻提取物对小鼠肺组织结构的影响与其VECF分泌作用的相关关系。 方法 昆明小鼠40只，随机分为五组，对照组及实验组（为四组），每组8只。对照组小鼠用生理盐水灌胃，实验组小鼠用不同剂量的蕨麻提取物每日灌胃，每只0.05ml/g。 实验小鼠一周后断颈处死。取肺组织用10%的福尔马林固定，脱水、透明浸蜡、切片、HE染色。肺组织肉眼及OLYMPUA-BX50显微镜下观察。结果 对照组小鼠肺组织无明显改变。实验组不同浓度的蕨麻提取物对小鼠肺组织的影响不同，Ⅰ组小鼠肺组织表面肉眼见不同程度的散在点状出血、显微镜下肺泡腔内见散在红细胞；Ⅱ组小鼠肺组织表面肉眼无明显异常，显微镜下肺泡腔内见散在红细胞及淡伊红色液体，两组均见肺泡壁毛细血管扩张充血及毛细血管内皮细胞呈不同程度的增生肥大。Ⅲ组及Ⅳ组的小鼠肺组织表面肉眼未见异常，显微镜下见肺泡壁毛细血管扩张呈不同程度的轻度充血。结论 不同剂量的蕨麻提取物对小鼠肺泡壁毛细血管内皮细胞的影响不同，肺泡壁毛细血管内皮细胞呈不同程度的增生肥大导致毛细血管通透性呈现不同程度的增加，表明可能与不同浓度的蕨麻提取物促进小鼠肺组织某些血管内皮细胞因子（Vascular endothelial cell factor VECF ）的分泌作用有关。
【关键词】 蕨麻；肺组织；血管内皮细胞
The relation between the effect of potentill anserine on pulmonary tissue structure of small rat and the VECF secretion function
【Abstract】 Objective To study different measure of potentill anserine extract to the relation between the effects of potentill anserine on pulmonary tissue structure of small rat and the VECF secretion function. Methods 40 Kunming small rats are divided into 5 groups at random. Control group and experimental group (4 groups)， each eight. The control group infuses stomach with normal saline; the experimental group infuses stomach with different measure of potentill anserine extract everyday， each 0.05ml/g. Test small rat breaking the neck to death after a week. The pulmonary tissue fixes with 10% formalin， dehydrates， and immerses the transparent examples in the wax， cuts into slices， HE dye. Pulmonary tissue is observed with naked eye and OLYMPUA-BX50 microscopically. Results The pulmonary tissue of control group small rat has no obvious change. The experimental group has various changes in the different density of potentill anserine extract. The small rat pulmonary tissue surface ofⅠgroup can see sporadic petechia of different degree; pulmonary alveolus cavity can see sporadic erythrocyte under the microscope. The small rat pulmonary tissue surface of Ⅱ group is no obvious abnormality by naked eye; pulmonary alveolus cavity can see sporadic erythrocyte and thin powder color liquid under the microscope. Two groups all have the capillary vessel of alveolar wall dilation and the endothelial cell hyperplasia and hypertrophy in different degree. The small rat pulmonary tissue surface of Ⅲ and Ⅳ group can’t see any abnormality; the capillary vessel of alveolar wall dilation appears a little hyperemia in different degree under the microscope.Conclusion Different measures of potentill anserine extract has different effects on endothelial cell of capillary vessel of small rats’ alveolar wall. Endothelial cell of capillary vessel of alveolar wall has hyperplasia and hypertrophy in different degrees; it leads to permeability of capillary vessel increasing in different extent. With the situation， different concentration of potentill anserine extract promotes vascular endothelial cell factor (VECF) of small rat’s pulmonary tissue secretion.