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| 全部作者： | 杨川 程桦 李焱 刘丹 王川 严励 傅祖植 |
| 第1作者单位： | 中山大学附属第2医院内分泌科 |
| 摘要： | The promising results in replacement of the beta-cell mass have been accomplished by beta-cells implanting. However, the demand for beta-cells is far greater than the supply. The transdifferentiation of stem cells into insulin-secreting cells could potentially provide a new help for beta-cell supplying. To determine whether the myoblast cell line C2C12 could be induced to transdifferentiate into insulin-secreting cells by pancreatic-duodenum homeobox-1 (PDX-1). A myoblast cell line (C2C12) was transfected by the PDX-1 constructing to overexpress PDX-1 protein and then treated with various concentrations of glucagon-like peptide-1 (GLP-1). The Expression of PDX-1 and insulin mRNA was determined by in situ hybridization and the intracellular insulin was determined by immunocytochemistry. The result shows that the PDX-1 transfected cells expressed more insulin and secreted more insulin than the vector transfected cells. We conclude that PDX-1 and GLP-1 might induce myoblasts to transdifferentiate into insulin-secreting cells. |
| 关键词： | GLP-1, glucagon-like peptide 1; ES, embryonic stem; DTZ 远程下载 (免费PDF全文) |
| 发表日期： | 2005年12月12日 |
| 同行评议： | 目前成体干细胞的可塑性时最为引人注目的，本文的研究进1步进行了检测，将为糖尿病的治疗提供诱人的临床前景。值得发表。 |
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