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VEGF在胃癌患者血清及肿瘤组织中的表达及其意义

2014-10-10 01:16 VEGF在胃癌患者血清及肿瘤组织中的表达及其意义

作者:周海茵，李 健，乔世铭，尹世萍

［摘要］ 目的 研究血管内皮细胞生长因子（VEGF）在胃癌患者血清及肿瘤组织中的表达情况，探讨其临床意义。方法 应用ELISA法对60例非肿瘤疾病手术患者和60例胃癌患者术前、术后血清VEGF水平进行定量测定，并应用免疫组化法测定肿瘤组织的VEGF。结果 胃癌患者术前血清VEGF高于对照组（P＞0.05）。两组术后较术前升高，肿瘤组术后较术前升高（P＜0.05）。肿瘤组术前血清VEGF水平、肿瘤组织中的VEGF与浆膜受累、淋巴结转移及临床病理分期有关。肿瘤组术后随访30～36个月中出现复发转移和死亡的患者，术前VEGF表达较其他患者升高（P＜0.05）。结论 血清VEGF水平及肿瘤组织中的VEGF与胃癌的浸润、转移有关，检测血清VEGF及肿瘤组织中的VEGF对胃癌浸润、转移程度和预后的判断有重要意义。

［关键词］ 胃肿瘤；微转移；血管内皮生长因子

Expression and significance of VEGF in serum and tumor tissue in gastric cancer

ZHOU Hai-yin，LI Jian，QIAO Shi-ming，et al.Department of General Surgery，The Central Hospital of Yangpu District，Shanghai 200090，China

［Abstract］ Objective To evaluate the clinical significance of vascular endothelial growth factor (VEGF) expression in serum and tumor tissue in gastric cancer patients.Methods The pre-operational and post-operational levels of serum VEGF were measured by ELISA kit in 60 cases of gastric cancer and 60 cases of non-carcinoma as the control group，the expression of tumor tissue VEGF was measured by immunohistochemical assay.Results The pre-operational level of serum VEGF in gastric cancer was higher than that in the control group(P＞0.05).The post-operational level of serum VEGF was increased in both groups，especially in the tumor group(P＜0.05).The expression of VEGF in serum and tumor tissue was positively correlated with tumor invasion depth，lymph-node metastasis and clinical pathological stage.A follow up of 30～36 months reveals the pre-operational level of serum VEGF of tumor recurrence and death in patients was higher than the others in the tumor group(P＜0.05).Conclusion The expression of serum VEGF and tumor tissue VEGF is closely correlated with invasion and metastasis of gastric cancer.Serum VEGF and tumor tissue VEGF assay may be used to pre-operatively predict invasion and metastasis and used to evaluate prognosis.

［Key words］ stomach neoplasm;micrometastasis;vascular endothelial growth factor

肿瘤的增殖和转移是血管新生依赖性的。在肿瘤血管生成过程中，血管内皮细胞生长因子（vascular endothelial growth factor，VEGF）是目前了解的功能最强大的特异性血管生长调节因子，各种转化生长因子、表皮生长因子、IL-6、IL-12、野生型P53及一些原癌基因均可上调VEGF产生促血管活性［1］，故VEGF可能为促血管活性因子的最后共同道路［2］。本研究通过对胃癌患者手术前后血清VEGF的定量测定及肿瘤标本中VEGF表达情况，探讨其与临床病理及肿瘤预后的关系。现分析报告如下。

1 资料与方法

1.1 一般资料 选择我院普外科2002年11月～2003年5月期间住院接受手术治疗的胃癌患者60例，其中男33例，女27例，年龄35～85岁，中位年龄68岁。其中Ⅰ期8例，Ⅱ期15例，Ⅲ期34例，Ⅳ期3例。对照组选择同期住院手术的非肿瘤患者60例。