反义寡核苷酸对视网膜色素上皮细胞中端粒酶逆
2015-01-08 02:15
导读：药学论文毕业论文，反义寡核苷酸对视网膜色素上皮细胞中端粒酶逆怎么写，格式要求，写法技巧，科教论文网展示的这篇论文是很好的参考：【关键词】 视网膜色素上皮；,寡核苷酸类,,反义；,端粒酶逆转录酶 Ef
【关键词】 视网膜色素上皮；,寡核苷酸类,,反义；,端粒酶逆转录酶
Effect of antisense oligonucleotides on telomerase reverse transcriptase gene expression and retinal pigment epithelium cell proliferation
【Abstract】 AIM: To investigate the telomerase reverse transcriptase (TERT) gene expression in retinal pigment epithelium (RPE) cells and the inhibitory effect of antisense oligonucleotides (ASODN) encoding TERT mRNA to gene expression and proliferation of RPE cells, so as to search for a new genetic therapy for proliferative vitreoretinopathy (PVR). METHODS: (1) Rabbit RPE cells cultured in vitro were detected for TERT expression by streptavidinbiotin complex (SABC) immunohistochemistry at several time points. (2) The ASODN and sense oligodeoxynucleotides (SODN) encoding TERT were delivered to the RPE cells at different concentrations, and then TERT expression was detected by immunohistochemistry. (3) Exposed to different concentrations of ASODN and SODN, proliferation activity and growthinhibitory rate of RPE cells were measured by MTT methods. RESULTS: TERT expression was markedly suppressed in the RPE cells treated with 5 and 10 μmol/L TERT ASODN. TERT ASODN significantly inhibited proliferative activity of RPE cells in a dosedependent manner. CONCLUSION: ASODN complementary to TERT mRNA at some concentration can sequencespecifically suppress TERT expression in RPE cells and cellular proliferative activity, and show a potential application in further experimental study for PVR genetic medication.
【Keywords】 retinal pigment epithelium; oligonucleotides, antisense; telomerase reverse transcriptase
【摘要】 目的：研究视网膜色素上皮(RPE)细胞中端粒酶逆转录酶(TERT)的表达及其反义寡核苷酸(ASODN)对其表达和细胞增生的抑制作用，为增生性玻璃体视网膜病变(PVR)探索基因治疗新途径. 方法：体外培养兔眼RPE细胞，在不同时间采用链霉亲合素生物素化过氧化物酶复合物(SABC)免疫组织
化学法检测TERT的表达；不同浓度的TERT ASODN和正义寡核苷酸(SODN)分别作用于体外培养的RPE细胞，采用免疫组织化学方法检测TERT的表达；四唑盐比色法(MTT)检测在不同浓度的TERT ASODN和SODN作用下RPE细胞生长活性及其生长抑制率. 结果：体外培养兔眼RPE细胞可表达TERT，在5，10 μmol/L ASODN作用下，TERT的表达明显受抑制；TERT ASODN能明显抑制RPE细胞增生活性，并呈剂量依赖性. 结论：一定浓度TERT ASODN能序列特异性地抑制RPE细胞TERT表达和增生活性，有望进一步用于PVR的治疗实验研究.
（转载自科教范文网http://fw.nseac.com） 【关键词】 视网膜色素上皮； 寡核苷酸类， 反义； 端粒酶逆转录酶
【中图号】 R322.91
0引言
端粒（telomere）是染色体末端高度保守的重复核苷酸序列，随着细胞的分裂而逐渐缩短，细胞趋向衰亡. 端粒酶是维持染色体长度和功能的重要物质，端粒酶的激活可使细胞持续增殖，避免衰老死亡. 在增生性玻璃体视网膜病变（proliferative vitreoretinapathy,PVR）的病理过程中，视网膜色素上皮（retinal pigment epithelial,RPE）细胞充当了极其重要的角色. 因此抑制RPE细胞的增生成为防治PVR的重要途径. Dickson等［1］提到端粒酶失活，端粒不断缩短可能是RPE细胞衰老的原因. 而增生细胞普遍存在端粒酶RNA结构，它可反向合成TTAGGG，加在DNA末端，从而使细胞的分裂不
