CEAmRNA论文
2013-11-02 01:02
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【关键词】 癌胚抗原 Different expression and clinical application of CEAmRNACE
【关键词】 癌胚抗原
Different expression and clinical application of CEAmRNACEAp system in benign and malignant ascites
【Abstract】 AIM: To understand the different expression of carcinoembryonic antigen mRNA (CEAmRNA)carcinoembryonic antigen protein (CEAp) system in benign and malignant ascites and to assess the value of CEAmRNACEAp for the diagnosis and differential diagnosis of benign and malignant ascites. METHODS: Ninetythree patients were examined, among whom 60 cases were with malignant ascites and 33 cases with benign ascites. The ascites were collected and used for CEAmRNA and CEAp detection. CEAmRNA was detected by reverse transcriptasenested primerspolymerase chain reaction (RTNPPCR) and CEAp was detected by magnetic antibody immunoenzymometric assay (MAIA). RESULTS: The positive rates of CEAmRNA and CEAp were 49 (81.7%) and 34 (56.7%) in the malignant ascites group and 1 (3.0%) and 3 (9.1%) in the benign ascites group.The positive rates of both CEAmRNA and CEAp in the ascites of patients with malignant ascites were significantly higher than those of benign patients (P
